Kinetic Basis for the Competitive Recruitment of TolB by the Intrinsically Disordered Translocation Domain of Colicin E9

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Competitive recruitment of the periplasmic translocation portal TolB by a natively disordered domain of colicin E9.

The natively disordered N-terminal 83-aa translocation (T) domain of E group nuclease colicins recruits OmpF to a colicin-receptor complex in the outer membrane (OM) as well as TolB in the periplasm of Escherichia coli, the latter triggering translocation of the toxin across the OM. We have identified the 16-residue TolB binding epitope in the natively disordered T-domain of the nuclease colici...

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ژورنال

عنوان ژورنال: Journal of Molecular Biology

سال: 2012

ISSN: 0022-2836

DOI: 10.1016/j.jmb.2012.01.039